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P. Ramanathan
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P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P1.04-080 - miR-326 Is Down-Regulated in Non-Small Cell Lung Cancer and Targets NFIB, a Lung Developmental Gene: A Pilot Study (ID 1326)
09:30 - 17:00 | Author(s): P. Ramanathan
- Abstract
Background:
Lung cancer is the leading cause of cancer mortality worldwide. Non-small cell lung cancer (NSCLC) is the most common subtype, accounting for about 80% of all lung cancers. miRNAs are small RNAs of 21-24 nucleotides in length, which play major role in cell proliferation and differentiation and their differential expression is known to be associated with various cancers including lung cancer. Role of miR-326 has been previously studied as a marker of bone metastasis in lung cancer. Moreover, we have previously shown that miR-326 plays a critical role in the epithelial to mesenchymal transition (EMT) by targeting transforming growth factor (TGF)-β1 and other members of TGF-β signaling pathway. The aim of present study is to check the expression and correlation of miR-326 and lung epithelial developmental gene nuclear factor IB (NFIB) in non-small cell lung cancer tissue samples as cancer metastasis is accompanied by EMT.
Methods:
We have examined eight pathologically confirmed non-small cell lung cancer cases. All patients were men and smokers with age ranged from 29 to 74 years (mean 54.6 years). Surgical resection was performed in all the cases which were either stage II or III. Histopathologically, 4 cases were squamous cell carcinomas, 3 were adenocarcinomas including one case of invasive mucinous carcinoma and one case was low grade mucoepidermoid carcinoma. RNA was isolated from fresh frozen tissue to check for miR-326 and NFIB levels by real time PCR. Protein expression was checked by immunohistochemistry (NFIB; 1:200; Abcam)) and in-situ hybridization (miR-326; Exiqon). Adjoining lung tissue served as normal control in each case.
Results:
Expression of both miR-326 and NFIB was found to be down regulated in non-small cell lung cancer tissue at both RNA and protein level (Fig 1A-C). Our in silico experiments identified a target site of miR-326 at the 3’UTR of NFIB gene; presumably it stabilizes the transcripts of NFIB (Fig 1D). Figure 1
Conclusion:
Our preliminary data suggests that miR-326 stabilizes the transcripts of NFIB in normal epithelial cells and maintain epithelial cell integrity. Dysregulation of miR-326 and NFIB in non-small cell lung cancer indicate that miR-326 and NFIB work synergistically and may contribute to the development of non-small cell lung cancer.
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P3.08 - Poster Session/ Thymoma, Mesothelioma and Other Thoracic Malignancies (ID 226)
- Event: WCLC 2015
- Type: Poster
- Track: Thymoma, Mesothelioma and Other Thoracic Malignancies
- Presentations: 1
- Moderators:
- Coordinates: 9/09/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P3.08-021 - Bronchopulmonary Carcinoid: A Developing Nation Cancer Center's Perspective (ID 2765)
09:30 - 17:00 | Author(s): P. Ramanathan
- Abstract
Background:
Bronchopulmonary carcinoids are low grade malignant tumours and increased incidence has been noticed worldwide. Our aim was to study preoperative characteristics, surgical approaches and outcome in bronchopulmonary carcinoid patients treated at a institutional cancer center.
Methods:
Twenty patients with bronchopulmonary carcinoids were surgically treated at department of surgical oncology, IRCH, AIIMS between December 2006 and December 2014. Preoperative variables, postoperative outcome and histopathological features were analyzed retrospectively. All patients underwent a detailed clinical evaluation followed by CECT of chest and bronchoscopy with biopsy of the suspicious lesion. After preoperative optimization, all patients were treated with upfront surgery. Adjuvant chemotherapy was given in patients with lymph nodal metastasis. Patients were followed up at three monthly interval with Clinical examination, chest X ray and serum chromogranin in cases of suspected recurrence. Because of cost factors Dotanoc scans were done only in cases with radiological suspicion of recurrence.
Results:
Patient’s median age was 40 years (range 18-62 years). All patients were symptomatic and median duration of symptoms before presentation was 18 months (range 6-72 months). Eight patients were treated with antitubercular drugs after presumptive diagnosis outside based on symptoms and Xrays. All the patients had lobar or main bronchial involvement which was detected on bronchoscopy and biopsy. Ninteen patients underwent complete pulmonary resection with mediastinal nodal dissection and surgical approach included eight pneumonectomies, four bilobectomies, five lobectomies, two sleeve resections. After a median follow up of 15 months, all patients were alive with no local recurrence or distant metastasis. On Histopathology all the resections margins were free of tumor and lymph nodal involvement was found in one patient.
Conclusion:
Delayed presentation and misdiagnosis are major concern in our scenario because of high prevalence of tuberculosis and despite a small number of cases our study emphasises the need for early bronchoscopy in patients with persistent symptoms. Aggressive surgical resection with technical approach of lung preservation may provide optimal survival results
