Author of

• 13580

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  P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 13632

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    P1.04-052 - TGF-β Induced EMT and Stem-Like Characteristics in Lung Cancer (ID 109)

    09:30 - 17:00  |  Author(s): Y.K. Kim
    • Abstract

    Background:
    TGF-β promotes tumor invasion and metastasis by inducing an epithelial-mesenchymal transition (EMT). EMT is often associated with acquisition of stem-like characteristics. In the present study, we investigated whether EMT induced by TGF-β could acquire stem-like characteristics in lung cancer.
    
    Methods:
    Normal epithelial cell line (BEAS-2B) and lung cancer cell lines (A549, H292, H226 and H460) were used in the study. These cell lines were incubated with 10 ng/ml of TGF-β for 3 days. Western blot was performed to analyze the expression of epithelial marker (E-cadherin) and mesenchymal markers (N-cadherin, vimentin, fibronectin and alpha-smooth muscle actin). Real-time RT-PCR and western blot were performed to analyze the expression of stem cell markers (CD44, CD133, CXCR4, ABCG2, CD117, ALDH1A1, EpCAM, CD90, Oct4, Nanog, SOX2, SSEA4, and CD166). Wound-healing assay, Matrigel invasion assay and sphere formation assay were used to assess functional characteristics of EMT and stemness acquisition.
    
    Results:
    TGF-β induced EMT and stem cell markers with variable degrees according to lung cancer cell lines. Most of the stem cell markers were increased by treatment with TGF-β except H460 cell line. Increased expression of mesenchymal markers was associated with the acquisition of stem cell makers. Migration, invasion and sphere formation were increased according to the expression of stem cell markers.
    
    Conclusion:
    TGF-β induced EMT was associated with acquisition of stem-like characteristics which was different according to lung cancer cell lines. Further studies are needed to investigate the signal mechanism of EMT and stemness acquisition.