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H. Nagano
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P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P1.04-021 - No MAML2 Gene Alteration Found in Ciliated Muconodular Papillary Tumor of Lung; Genetic Difference from Mucoepidermoid Carcinoma (ID 490)
09:30 - 17:00 | Author(s): H. Nagano
- Abstract
Background:
MAML2 (mastermind-like 2 (Drosophila)) gene, a transcriptional coactivator for NOTCH proteins, is known to be involved as a part of fusion gene (MECT1-MAML2) which is found in mucoepidermoid carcinoma (MEC) both of salivary gland and pulmonary origin. Ciliated muconodular papillary tumor (CMPT) is sharing morphologic features with mucoepidermoid carcinoma, at least in part, i.e. consist of mixture of mucinous and squamous epithelium. To determine whether these morphological mimics can share the molecular alterations, we evaluated MAML2 rearrangement of CMPT by FISH.
Methods:
Five cases of CMPT was recruited from pathologic diagnostic records between 2005 to 2014. Morphological assessment was done on routine HE stained slides of whole tumor specimen. Representative area was selected and submitted to FISH analysis. Fluorescence in situ hybridization (FISH) using break apart type MAML2 gene probes was performed on FFPE specimen. The break apart signal percentages on separated tumor nuclei was counted on the captured images of digital fluorescence microscope. 100 nuclei was counted in each cases. More than 30% of break apart signal is considered as positive result.
Results:
All five CMPTs were reviewed and confirmed the diagnosis on HE stained slides. These cases included 4 male, 1 female, were mean age of 71 years-old (range 60-83). There were three incidental cases which were patients with one primary lung adenosquamous carcinoma and two metastatic cancer (one colon cancer, one liposarcoma). All of five CMPT resulted negative for MAML2 break-apart FISH.
Conclusion:
These results indicated that CMPTs do not share the molecular alteration of MAML2, which is commonly detected in mucoepidermoid carcinoma of lung. In conclusion, CMPT is a distinct tumor or tumor-like lesion, does not related to MEC. Although, it is still uncertain whether CMPT is a true neoplastic lesion with multi-lineage differentiation potential or a reactive process with extensive epithelial proliferation.
