Author of

• 10583

  +

  P1.06 - Poster Session 1 - Prognostic and Predictive Biomarkers (ID 161)

  • Event: WCLC 2013
  • Type: Poster Session
  • Track: Biology
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/28/2013, 09:30 - 16:30, Exhibit Hall, Ground Level

  • 10608

    +

    P1.06-025 - Determination of the activity of lysosomal enzymes and protease inhibitors is useful in the diagnostics of lung cancer. (ID 1977)

    09:30 - 16:30  |  Author(s): G. Drewa
    • Abstract

    Background
    Lysosomal proteolytic enzymes play an important role in carcinogenesis and metastasizing processes. Activation of lysosomes may result in an increased exfoliation of cancer cells, which in vivo may promote metastatic progression. It was also observed that increased activity of lysosomal enzymes is connected with an increased permeability of cellular membranes and vascular endothelium, in turn associated with promoting metastasizing, also in lung cancer.

    Methods
    We evaluated the activity of selected lysosomal enzymes and one of protease inhibitors in serum, lung parenchyma and lung tumour, in 41 patients operated on with radical intent due to non-small cell lung cancer (NSCLC). Control group consisted of 44 healthy individuals. Cathepsin D, acid phosphatase, arylsulfatase and alpha-1-antitrypsin serum concentration was measured in patients before surgery, and on day 7, and 30 after operation. The concentration of these enzymes was also measured in the tumor and in healthy lung parenchyma. Obtained results were compared with control group, where concentration of enzymes was measured only in serum.

    Results
    In NSCLC patients an elevated serum concentration of cathepsin D (p<0.001), acid phosphatase (p<0.001) and arylsulfatase (p<0.001) was observed, compared with the control group. Serum concentration of acid phosphatase (p=0.033) and arylsulfatase (p=0.004) was elevated in patients with metastases to regional lymph nodes. Concentration of acid phosphatase (p<0.001), arylsulfatase (p<0.001) and alpha-1-antitrypsin (p<0.001) was higher in pulmonary tumor than in the healthy lung parenchyma. Concentration of acid phosphatase (p=0.002) and arylsulfatase (p<0.001) in pulmonary tumor was also elevated in patients with metastases to regional lymph nodes. In lung cancer patients, postoperative concentration of acid phosphatase and arylsulfatase decreased significantly, as comperative values. Figure 1 Figure 1. Chosen biomarkers activity comparison. (A) Comparison of cathepsin D (Cat D) activity in NSCLC patients with (N1+N2) and without (N0) lymph node metastases at baseline, POD 7 and POD 30. (B) Comparison of arylsulfatase (AS) activity in NSCLC patients with (N1+N2) and without (N0) lymph node metastases at baseline, POD 7 and POD 30. P values for each comparisons were obtained with Mann-Whitney U tests; POD, post-operative day.

    Conclusion
    Serum concentration of cathepsin D, acid phosphatase, arylsulfatase and alpha-1-antitrypsin is useful in the diagnostics of NSCLC. Moreover, serum acid phosphatase and arylsulphatase concentrations are useful in postoperative monitoring of these patients.