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L. Zhang

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    P1.02 - Poster Session 1 - Novel Cancer Genes and Pathways (ID 144)

    • Event: WCLC 2013
    • Type: Poster Session
    • Track: Biology
    • Presentations: 1
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      P1.02-022 - Downregulation of PAX6 by siRNA Inhibits Proliferation and Cell Cycle Progression of Human Non-Small Cell lung Cancer Cell Lines (ID 205)

      09:30 - 16:30  |  Author(s): L. Zhang

      • Abstract

      The transcription factor PAX6 is primarily expressed in embryos. PAX6 is also expressed in several tumors and plays an oncogenic role. However, little is known about the role of PAX6 in lung cancer.

      In this study, the function of PAX6 in lung cancer cells was evaluated by small interfering RNA-mediated depletion of the protein followed by analyses of cell proliferation, anchorage-independent growth, and cell cycle arrest. The PAX6 mRNA level in 53 pairs of tumors and corresponding matched adjacent normal tissues from non-small cell lung cancer patients and lung cancer cell lines was detected by real-time PCR.

      Suppression of PAX6 expression inhibited cell growth and colony formation by A549 and H1299 cells. The percentage of cells in G1-phase increased when PAX6 expression was suppressed. The cyclin D1 protein level, as well as the pRB phosphorylation level, decreased as a result of PAX6 down-regulation. PAX6 mRNA was highly expressed in lung cancer tissue and lung cancer cell lines.

      Our data support the hypothesis that PAX6 accelerates cell cycle progression by potentiating G1-S progression.