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H. Kim

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    P1.02 - Poster Session 1 - Novel Cancer Genes and Pathways (ID 144)

    • Event: WCLC 2013
    • Type: Poster Session
    • Track: Biology
    • Presentations: 1
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      P1.02-017 - NLBP (novel-LZAP binding protein) promotes the proliferation of lung adenocarcinoma cells through the regulation of p120 catenin (ID 1873)

      09:30 - 16:30  |  Author(s): H. Kim

      • Abstract

      NLBP (novel-LZAP binding protein, also known as KIAA0776 or Maxer) was originally indentified as a reciprocal binding partner of LZAP. LZAP possesses dual functionality as a tumor suppressor and an oncogene in human cancers. Despite its strong association with LZAP, the biological roles and underlying molecular mechanisms of NLBP remain unknown. Recent studies indicating a possible link between p120 catenin (p120ctn) and NLBP in tumorigenesis.

      We examined NLBP expression in 29 non-small cell lung cancers using immunohistochemical staining and immunoblotting, as well as an in vitro analysis of NLBP activity during cell proliferation in lung cancer cell lines. We next examined whether p120ctn plays a role in the molecular mechanism underlying NLBP activity

      In this study, we found that NLBP expression was increased in human lung adenocarcinomas, particularly early-stage adenocarcinomas, compared to squamous cell carcinomas. Furthermore, the overexpression of NLBP in H1299 cells resulted in an increase in cell proliferation. We next identified p120ctn as a novel NLBP-binding protein, and identified the reciprocal binding regions of these proteins in lung cancer cell lines. We also demonstrated that NLBP promotes cell proliferation by regulating the stability of p120ctn. This effect is mediated through the inhibition of ubiquitination, which occurs as a result of NLBP binding to p120ctn, leading to an increase in protein stability. Figure 1

      In conclusion, the data presented here show that NLBP may act as a novel oncogene in the early stages of lung adenocarcinoma, and may promote cell proliferation in lung adenocarcinoma through interactions with p120ctn. The interaction between NLBP and p120ctn provides new implications for interplay between signaling pathways and protein networks in tumor development