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P1.01 - Poster Session 1 - Cancer Biology (ID 143)
- Event: WCLC 2013
- Type: Poster Session
- Track: Biology
- Presentations: 1
- Coordinates: 10/28/2013, 09:30 - 16:30, Exhibit Hall, Ground Level
P1.01-017 - The retain of extrapulmonary administered colloidal carbon in the mediastinal lymphnodes of Wistar rat (ID 115)
09:30 - 16:30 | Author(s): I. Kocakova
Model of metastatic spreading of extrapulmonary malignancy into mediastinal lymhnodes.
Subcutaneous administration of 0,4 ml of the ink (compound from black carbon dispersed on colloid casein solution i.e. colloidal carbon / KOH-I-NOOR Hardtmuth Ltd., Praha, CZ) diluted with sterile saline solution 1:3 was performed under i.m. general anesthesia (diazepam, xylazine, ketamine) in 42 outbred Wistar rats (An-Lab Praha, CZ) divided into 7 groups according the application sites: retroperitoneum, testis / ovary, hind leg, front leg, thoracic wall, pleural cavity. On seventh day after the administration all rats were euthanatized, the thoracic wall was opend and mediastinal lymphnodes were detected macroscopically, taken out, and preserved in 18% formalin. Paraplast fixation and the hematoxyline-and-eosine staining were used for histological examination by 40-times and 100-times magnification. The samples with monocyte- macrophage system cells containing colloidal carbon (CC) in the mediastinal lymphnodes (MLNs) were considered positive. Two statistical methods were used to determine the probability of model cell transport and retention. First is based on random error theory and consists of mean values, standard deviations and confidence intervals. Second analysis calculated the confidence intervals of probabilities using F-distribution. Programs Matlab 2012 (MathWorks) and Microsoft Office 2010 Excel (Microsoft Corp.) were used for the statistical analysis. FIG 1 The sites of ink administration 1 2 3 4 5 6 7 1 - thoracic wall, 2 - front leg, 3 - pleural cavity, 4 - retroperitoneum, 5 – testis, 6 - hind leg, 7- ovary
Macrophages containing carbon particles were found in MLNs of all groups of animals, at least in one animal of each tested group. Positive lymphnodes were found in 4/6 (66.7%) of animals after paraovarial, paratesticular and pleural cavity application and in 2/6 (33.3%) with retroperitoneal and distal part of hind leg application. In animals injected in distal part of front leg and in thoracic wall it was 1/6 (16.7%) with positive nodes. Statistical analysis showed significant retain of CC administered into different parts of the periphery of the body on the 5% significance level.
The transport of CC with the monocyte macrophage system cells from very different parts of the body to the mediastinal lymph nodes of Wistar rats and their persistence there after seven days has been proved on the 5% significance level. That indicates the relation between mediastinal lymphnodes and alien loading of the body.